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SIAIS Analytical Chemistry Platform

Analytical platform is a core facility funded by SIAIS-Shanghaitech University. The goal of the platform is to provide biomolecules characterization services to scientists at Shanghaitech University and external clients globally.The platform is equipped with state-of-the-art facility including two Orbitrap Fusion MS, one QTOF MS, one 500M NMR, one two-D UPLC, one GCMS, etc. A wide range of software tools including Proteome Discover, Maxquant, MASCOT, SCAFFOLD, HDExaminer, PEAKS, Biopharma, and SimGlycan are also available for the following Database searching and data visualization. The platform staff work closely with SIAIS bioinformatics team to provide statistical solution to our clients.

 

免疫化学所分析化学平台隶属于上??萍即笱?,致力于为上科大课题组及其他科研机构提供高质量的生物分析(主要是生物质谱分析)最信誉的买球APP推荐最信誉的买球APP推荐。平台配备的设备包括:两台超高分辨质谱仪Orbitrap Fusion(Thermo Scientific)最信誉的买球APP推荐,两台nanoLC(Thermo Scientific EASY1200)最信誉的买球APP推荐最信誉的买球APP推荐,一台微升液相(Thermo Scientific U3000)一套氢氘交换系统(Leaps)最信誉的买球APP推荐,一台QTOF-MSAB Sceix 4600,一台二维液相(Aglient 1290)最信誉的买球APP推荐最信誉的买球APP推荐。平台配备一系列数据分析软件最信誉的买球APP推荐,包括PD2.2(Thermo Scientific)最信誉的买球APP推荐,Maxquant, MASCOT, SCAFFOLD, HDExaminer, PEAKS, Biopharma, SimGlycan最信誉的买球APP推荐,用于分析不同的质谱数据。

 



Major instruments

主要设备

nLC-Orbitrap Fusion MS (Thermo Scientific)

纳升液相-Orbitrap Fusion三合一质谱联用仪

The instrument is equipped with nano UPLC and Easyspary source to ensure the best HPLC separation resolution. The instrument is used to identify and quantify a variety type of samples such as single gel band sample and whole cell lysate sample.

该设备为EASY1200纳升液相与Fusion质谱联用最信誉的买球APP推荐最信誉的买球APP推荐,配有EASYSPRAY离子源最信誉的买球APP推荐,主要用于蛋白组学分析最信誉的买球APP推荐最信誉的买球APP推荐,样品种类涵盖胶条、细胞/组织裂解液等多种形式最信誉的买球APP推荐。


HDX-Orbitrap Fusion (Thermo Scientific)

氢氘交换-Orbitrap Fusion三合一质谱联用仪
 The instrument is coupled with Leap robot sample preparation system. The instrument is used to identify the binding site of a drug or antibody (epitope mapping) on its target protein by using Hydrogen/Deuterium Exchange technique.

该设备配有氢氘交换自动反应-淬灭-进样系统(LEAP-微升液相U3000(Thermo Scientific)Fusion质谱仪最信誉的买球APP推荐,用于氢氘交换研究最信誉的买球APP推荐最信誉的买球APP推荐最信誉的买球APP推荐。


Hybrid Quadrupole-Time-of-Flight MS (Sciex 4600)

四级杆-飞行时间质谱
 The instrument is coupled with SHIMADZU UPLC system to improve peak resolution and throughput. The instrument is mainly utilized to measure exact mass of synthetic small molecules, oligonucleotides, and other biomolecules.

该设备配有岛津UPLC系统和 AB Sciex QTOF4600质谱仪最信誉的买球APP推荐,用于化学小分析分析最信誉的买球APP推荐、寡核苷酸分析和其他一些生物分子分析最信誉的买球APP推荐。


500M NMR spectrometer (Bruker)

500M核磁
 Small molecule structure characterization

小分子结构分析


MALDI-TOF/TOF-MS (Sciex, 5800)

基质辅助激光解吸离子化-飞行时间质谱
 High throughput MS determination

用于高通量质谱分析


2-D UPLC system (Agilent)

二维液相系统

用于二维/一维液相分析

 

BiacoreT200 (GE)

蛋白亲和活性仪

The instrument is used to detect biomolecular interactions in real-time without labeling, such as protein-protein, DNA-protein, DNA-DNA, etc. The affinity, kinetics and thermodynamics of biomolecular binding can be quantitatively calculated.

该设备可用于实时检测各种生物分子间的相互作用最信誉的买球APP推荐,蛋白质-蛋白质最信誉的买球APP推荐最信誉的买球APP推荐,DNA-蛋白质最信誉的买球APP推荐最信誉的买球APP推荐,DNA-DNA等。 无需标记最信誉的买球APP推荐,定量计算生物分子结合的亲和力、动力学和热动力学。

 

Chirascan-plus Circular Dichroism Spectrometers (Applied Photophysics)

圆二色谱仪

The instrument utilize the circular dichroism of proteins and the difference in absorption of left and right circularly polarized light by asymmetric molecules to perform structural analysis. It can be used for protein folding, protein conformation study, DNA /RNA reaction, optical active material purity measurement, drug quantitative analysis.

该设备利用蛋白质的圆二色性及不对称分子对左右圆偏振光吸收的不同来进行结构分析最信誉的买球APP推荐∽钚庞穆蚯駻PP推荐?捎糜诘鞍字收鄣?最信誉的买球APP推荐最信誉的买球APP推荐最信誉的买球APP推荐最信誉的买球APP推荐、蛋白质构象研究,DNA /RNA 反应最信誉的买球APP推荐最信誉的买球APP推荐,光学活性物质纯度测量最信誉的买球APP推荐最信誉的买球APP推荐,药物定量分析最信誉的买球APP推荐。


MicroCal PEAQ-ITC (Malvern)

等温滴定微量热仪

The instrument is used to detect molecular interactions between small molecules, proteins, antibodies, nucleic acids, lipids, and other biological molecules.

该设备应用于表征小分子、蛋白质、抗体最信誉的买球APP推荐、核酸最信誉的买球APP推荐、脂质和其他生物分子的分子间相互作用最信誉的买球APP推荐。



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